"Hong, Minsun" <compania at donald.hanho.co.kr> wrote:
>Hello..
>>i'm trying to find out that my recombinant protein which is secreted by
>S. cerevisiae, is multimer or monomer in culture medium.
>>would you show my applicable methods?
>and if it will be mutimer, is it possible to purify it as it is?
Get in touch with somebody who has an analytical ultracentrifuge. They can
determine the molecular weight in a single run, at least if the sample is
monodisperse.
Both gel chromatography and rate zonal centrifugation in sucrose or glycerol
gradients are getle methods to separate proteins by MW.
