I have been trying for some time now to overexpress my protein in a
number of strains that are auxotrophic for proline but without sucess.
A number of stains do not transform at all. Other strains express very
poorly. DOes anyone out thre have expeience in dealing with these problems.
If so please email me at tnrk1 at cus.cam.ac.uk
cheers
Tom
