Dear Dr. protein:
I cloned a protein with His-tag at C-end. After I purified this perotein
by Ni-column, SDS-PAGE gel show that the protein was degraded.What is
the reason? Is there any other methods which can solve this problem
except using proteinase inhibiter coke-tail or EDTA in the buffer?
Thank you for your help!
qiu at orion.cmc.uab.edu
