John Tomaszewski schrieb:
> >Dear everyone,
> >
> >I am trying to detect the low molecular weight(8KD) protein using
> >Tris-Tricine SDS-PAGE. But when I dried the gel, the gel was easily
> >crashed.
>> I run a 16.5% gel (from 29:1 acryl/bis stock) for 6 and 7.5KD
> proteins. After destaining in 40% MeOH/10% HOAc I give it about an
> hour
> in 5% MeOH/7% HOAc and it dries just fine under heat and vacuum.
>> John T.
>> ********************************************************************
> ** ** ** ** * ** DEPARTMENT OF CHEMISTRY **
> ** ** ** ** * ** tomasz at protein.chem.washington.edu **
> ** ** ** ** * ** (206) 616-2993 **
> ** ***** ***** JOHN TOMASZEWSKI **
> ********************************************************************
Hi John,
we had the same problem with 15 % gels. We put it in 10 % glycerine for
30 min and then dried it.
Reinhard
