IUBio

Buffer at pH 3-4?

Achim Recktenwald reckteac at ibex.ca
Tue Jul 29 07:53:54 EST 1997


akhan at unix1.sncc.lsu.edu wrote:
> 
> Hello.  Could anyone suggest a buffer with pKa of 4 or below that can be
> used in anion exchange chromatography (i.e. it would not produce anions).
> Thank you.  Anwar Khan.


The lowest I could find was N-methyl-piperazine with a pKa 0f 4.75.
Pharmacia recommendsa it as a good one in one of their pamphlets.

Achim
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Achim Recktenwald, PhD
IBEX Technologies, Inc.
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