akhan at unix1.sncc.lsu.edu wrote:
>> Hello. Could anyone suggest a buffer with pKa of 4 or below that can be
> used in anion exchange chromatography (i.e. it would not produce anions).
> Thank you. Anwar Khan.
The lowest I could find was N-methyl-piperazine with a pKa 0f 4.75.
Pharmacia recommendsa it as a good one in one of their pamphlets.
Achim
__________________________
Achim Recktenwald, PhD
IBEX Technologies, Inc.
5485 rue Pare
Montreal, Quebec, H4P 1P7
Canada
Fax : (514) 344-8827
email: achim at ibex.ca
The usual disclaimers apply.