Wildwind (swvong at sciborg.uwaterloo.ca) wrote:
: Hello,
: I have extracted protein for 1-D electrophoresis in plant tissue and
: have had no problems in the past. However, recently (in seed tissue) I
: have had severe streaking throughout the entire gels (not just in the
: lanes) following staining, making detection of bands impossible. I asked
: several co-workers who believe it may be polysaccharides in the sample
: buffer. Does anyone have any suggestions on how to fix this problem? It
: would be appreciated.
: Thanks
Hello there,
I had a similar problem a few months ago.
The culprit for the streaking turned out to be
the dirty gel running buffer.
Try running the gel with a fresh batch of gel running buffer.
Hope this helps.
InYup
