In article <33CD6762.41C6 at arlo.medcor.mcgill.ca> li2 at ARLO.MEDCOR.MCGILL.CA (li2) writes:
>I am doing fluorescence studies of the unfolding process of the protein
>by guanidine hydrochloride denaturation. Could anyone help me with the
>reaction condition ie. temperature, pH of guenaidine hydrochloride,
>reaction time. I will be greatly appreciated.
>Thanks
>Jun
What protein? That determines what temperature/pH it's happy at.
10, 15, 20, or 25 degrees Celsius is common. Try pH 7. Use at least
6 M Guanidine if you don't know where the transition will be.
