Hello,
I have extracted protein for 1-D electrophoresis in plant tissue and
have had no problems in the past. However, recently (in seed tissue) I
have had severe streaking throughout the entire gels (not just in the
lanes) following staining, making detection of bands impossible. I asked
several co-workers who believe it may be polysaccharides in the sample
buffer. Does anyone have any suggestions on how to fix this problem? It
would be appreciated.
Thanks
