Hello,
I have sequenced a cDNA and I am not sure to have the N-terminal
extremity of my protein because my Kozak's sequence is not good:
gccacttcggATGg, futhermore, the 1040 bases before this sequence seem
to be no codant because of the presence of numerous codon stop in the
three ORF and strech of A or T. My protein is a homologue of a family
of transmembran protein and I have search a peptide sequence signal to
know if I have the N-terminal extremity or not. This is the first
amino acids of my sequence :
MERMFEARWVNSSTVECVHVLLHTSEKSHLFPINLQLKDRPDRFI.....
What do you think about it, Do I have a peptide signal?
If no, do you know transmembran protein localizes on the plasma
membran without this sequence?
