> Hi!
> I am trying to purify a protein using C18 RP-HPLC. Unfortunately it
> precipitates when sample mixed with A buffer (0.1% TFA in water).
> Is there another good buffer system (pH>6) for the protein separation on
>
You can try phosphate buffer (say 10-20 mM, pH 2.0, 4.4 and
6.5). Silica is notorious for not liking Alkaline pHs so
I would not go above pH 6.5.
Regards,
John
_________________________________________________________
John Marcus Marcus at tpp.uq.edu.au (Dr J.Marcus)
Cooperative Research Centre for Tropical Plant Pathology
5th Level John Hines Building
University of Queensland
St. Lucia, QLD 4072
AUSTRALIA
Fax: 61-7-3365-4771
Phone: 61-7-3365-4764
