I am overexpressing an enzyme in E. coli for kinetic analysis. Can the
E. coli cell density at the time of IPTG induction affect the specific
activity of the enzyme eventhough I purify the enzyme before I assay it?
I ask this because my preps seem to have differing specific activities
and its not due to pipette or protein assay errors.
Brian
bmark at cc.umanitoba.ca
