Hi everybody,
I am working with enzymes using polysaccharides as substrate. Each
polysaccharide molecule contains several 'substrate-sites' at which it
is cut into subsequently smaller fragments. With all likelihood the
affinity of the enzymes for these fragments differs, e.g., the Km and/or
Vmax-value is not the same, as for the undigested polymer.
I am now looking for publications describing the enzyme kinetics and
experiments concerning the enzyme mechanism of these kind of enzymes.
Since there are many enzymes with a similar behavior, e.g., amylases,
DNA restriction enzymes, and so on, I am hoping that there is some
literature availabe.
Any help would be appreciated.
Thanks a lot in advance,
Achim
P.S. Our service-provider seems to have occasional problems with the
news-server. Please send me an email-copy of any answer.
___________________________
Achim Recktenwald, PhD
IBEX Technologies, Inc.
5485 rue Pare
Montreal, Quebec, H4P 1P7
Canada
achim at ibex.ca
