Dear Public_Access_Machine,
With regards to your posting on the NEB Intein system, what kind of bugs
were you using and what kind of yields were you getting? We're a
structural biology lab and I was never able to get anything that looked
like good induction. To be honest, I also never went ahead and purified
the protein with the chitin resin, so I can't really say what my yeilds
might have been but we need crap loads of protein from preferably
minimal media. So far, I have tried expressions in LB in the bacterial
strains BL21(DE3), TB1 and DH5a. Any suggestions, hints or freebees
would be greatly appreciated...
Randall C Willis, Researcher
Biochemistry Research
Hosp for Sick Children
3522-555 University Ave
Toronto, ON
M5G 1X8 CANADA
416-813-5933 (ph) -5022 (fax)
willis at gandalf.psf.sickkids.on.ca
