In article <32EDFB6D.2792 at nih.gov>, rlevine at nih.gov wrote:
> As the responses have pointed out, MALDI-TOF conditions always (my
> experience) lead to dissociation of non-covalent subunits. However, it
> seems to me that a simple solution is to add a cross-linking reagent to
> your sample, locking it in the correct quaternary structure. After
> adding a quenching agent to take up the excess cross-linker, just run
> the MALDI.
>> Has anyone tried this?
>> Rod Levine
> NIH
Yes, this has been done.
Farmer, T. B., and R. M. Caprioli. 1991. Assessing the multimeric states
of proteins: studies using laser desorption mass spectrometry. Biol. Mass
Spec. 20:796-800
