As the responses have pointed out, MALDI-TOF conditions always (my
experience) lead to dissociation of non-covalent subunits. However, it
seems to me that a simple solution is to add a cross-linking reagent to
your sample, locking it in the correct quaternary structure. After
adding a quenching agent to take up the excess cross-linker, just run
the MALDI.
Has anyone tried this?
Rod Levine
NIH
