Andreas is right!
One caveat:
Thaw and MIX THOROUGHLY before decanting your aliqout for addition of
TEMED/APS. You can use a microwave oven for accelerated thawing, but be
careful to keep the temperature of the stock solution from getting above
40 degrees C!
Len Ornstein
<http://www.pipeline.com/~lenornst/DiscElectrophoresis.html>
>Andreas Strecker wrote:
>> On 12 Jan 1997 13:40:33 GMT, "Thorsten Schmidt"
> <Thorsten.Schmidt at rz.ruhr-uni-bochum.de> wrote:
>> >Dear reader!
> >
> >I make many Western blots and SDS-PAGE-gels.
> >
> >For each gel, I have to mix
> >- Acrylamid-Mix (29 % acrylamid / 0,8 % bis-aa)
> >- Tris-Buffer
> >- Water
> >- 10 % SDS
> >- TEMED
> >- 10 % APS
> >
> >to pour a 3% and a 10% gel.
> >
> >Is there anything to be said against preparing a 3 % and a 10 % gel-mix
> >by mixing the componets (except for APS & TEMED) in advance?
> >
> >It would minimize time by just adding TEMED & APS to a aliquot!
> >Or isn´t it good to store such a mix for a longer time?
> >
> Hi Thorsten!
>> There simply is NOTHING to say against storing premixed gel solutions
> as long as you keep them in a freezer (-20 degr.). You should be able
> to keep them at least for a couple of months. And it improves the
> reproducibility of your gels (if that is important for you). We have
> made good experience with it.
>> Andreas
