IUBio

Labelling protein markers

Ron Tate rtate at bmb-fs1.biochem.okstate.edu
Fri Jan 24 11:49:43 EST 1997


Antonio Rodriguez Franco wrote:
> 
> Is there any reliable protocol to covalently label protein markers to
> use it in native and denaturing PAGE?.
> I mean that kind of label that let you see how your electrophoresis is
> going on, and let your proteins be  transferred to the membranes for
> Western analyses without needing further staining.
>
Antonio, 
	I use prestained molecular weight markers for this.  Bio-Rad and Novex
make some of these as well as others I'm sure. Novex I think has a
pretty good assortment of mixes to choose from.
-- 
>>>>>>--------------------------->
Ron
Tate                                                                   
Lab of Franklin Leach
Dept. of Biochem. & Molecular Biology
Oklahoma State University 
rtate at bmb-fs1.biochem.okstate.edu
(405) 744-9326
---------------------------------------------



More information about the Proteins mailing list

Send comments to us at biosci-help [At] net.bio.net