There have been only a few papers in the literature on detecting specific
non-covalent complexes by MALDI, and at this point in time it is
probably best to consider MALDI only if one is working with a
mass spectrometry group specializing in that kind of research. Moreover,
the conditions under which a MALDI analysis is performed are usually
denaturing. At this point in time, reviewers would probably not readily
consider a MALDI observation of a non-covalent complex sufficient
proof on it's own.
Electrospray ionization mass spectrometry is rapidly emerging as an
excellent method for determining stoichiometries of non-covalent
complexes. The analysis needs to be performed under non-denaturing
conditions--different from how routine work is usually done.
Rachel Ogorzalek Loo
2560 MSRB II
Ann Arbor, MI 48109
