MALDI-TOF analysis is suitable to identifying whether a protein is a
homodimer or a homotrimer?
I have a small DNA-binding protein(6,100 dalton) quite well purified.
Through size exclusion HPLC gel chromatography, I found its effective
molecular weight of the protein in the absence of its binding DNA
sequence is about 19,000 dalton, close to homotrimer.
But I am not sure that it is a trimer based on only the result becuase I
didn't ever hear about homotrimer DNA binding protein; so I need some
additional experiments confirming this fact.
Thank you for your time and expertise,
Lee, Ji Hyun
