I was having problems recently with the samples of my Tris-Tricine
SDS-PAGE/Westerns streaking vertically. After checking the usual suspects
(old sample buffer, precipitated sample, etc.) I found a few protocol
books mentioning that turning the current down sometimes helps this
problem. When I did that, it did drastically decrease the streaking, but
really increased the running time. I was wondering if anyone knew what
phenomenon is causing this streaking, and is there some other way of
correcting it so I can run my gels faster again.
--
Scott McMahan
mcmahan at oncology.wisc.edu
