APS & TEMED in acid gels

[Louis Hom]

In article <32E3E5D5.1F70 at topaz.microbio.uab.edu>,
Matthew Parker  <parker at topaz.microbio.uab.edu> wrote:
>Sure, dump some more in there, shouldn't hurt. How much do you use per
>ml of gel?

For the resolving gel (15%, 12.5ml) I use 125ul 10% APS and 33ul TEMED.  For 
the stack (4%, 5ml) I use 90ul APS, 25ul TEMED. 
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_______________________________________________________________________________
Lou Hom >K '93	
lhom at nature.berkeley.edu  
http://www.ocf.berkeley.edu/~lhom