Does anyone know of a simple method to precipitate and then count 35-S
labelled proteins transcribed in rabbit reticulocyte lysate? I have tried
regular TCA precipitation (spot on Whatman, 10% TCA, then washing) but the
counts don't agree with the gel. There is a method in the Promega manual that
involves treating with NaOH/H2O2, then precipitating with 25%TCA/2% casamino
acids, followed by vacuum filtration on to glass filters. Does anyone know if
this method is good?
Thanks.
Nigel