In article <ammacleo-2001971621020001 at kalmarlab2.mbb.sfu.ca>, ammacleo at sfu.ca (Alasdair MacLeod) wrote:
# I am trying to figure out the best way to accurately determine the
#concentration of a mutant enzyme and can't use an active-site titrant.
#I am leary of dye-binding and the Lowry method to accurately quantify this
#protein. Is anyone else of the opinion that absorbance OD280 (ex. method
#of Scopes) is any better?
Well, if you have both proteins in pure form AND mutation does not replace
aromatic aa (esp. tryptophan), this is, IMHO, the best way to go.
- Dima
