So I'm running these HOAc-urea polyacrylamide gels (15% resolving, 4%
stack) and each part takes >1hr to polymerize at the concentrations of APS
and TEMED that I currently use. Will I mess things up by increasing the
APS & TEMED concentrations so that polymerizatin occurs more on the order
of 30 minutes instead? I won't be transferring or eluting the proteins
from the gel for anything.
--
_______________________________________________________________________________
Lou Hom >K '93
lhom at nature.berkeley.eduhttp://www.ocf.berkeley.edu/~lhom
