Disulphide bonds

[Tom Duncan]

Richard P Grant wrote:
> 
> We have a domain that is a bit capricious - it tends to precipitate
> and is reasonably unstable in solution.  We'd like to stabilize it and
> I thought of introducing a judicious S-S by sdm.  Does anyone know if
> this has been done, or is there a reference that I might look up?
> 
> TIA,
> Richard
> 
> --
> Richard P. Grant  MA  DPhil        University of Oxford
> Nuffield Department Obstetrics and Gynaecology      FFPGP
> http://users.ox.ac.uk/~lady0266    http://www.molbiol.ox.ac.uk/~rgrant
> --------------# The Internet is _full_. Please go away #--------------

One good article I have is

	"Probing the Determinants of Disulfide Stability in
	Native Pancreatic Trypsin Inhibitor". Goldenberg et al., 1993.
	Biochemistry _32_, 2835-44.

It also references a number of articles that introduced disulfides in
attempts to stabilize proteins. Good luck.
-- 
	Thomas M. Duncan
	Dept. Biochemistry & Molecular Biology
	SUNY Health Science Center
	750 E Adams St,	Syracuse, NY 13210
	Email:	duncant at vax.cs.hscsyr.edu