protein-SDS interactions?

[David Clarke]

Hi,

I've a question I'm hoping that someone will be able to answer. I have mutated the 
trans-membrane domain of a protein and I've found that one relatively conservative 
mutation, isoleucine->methionine, rapidly alters the mobility of the protein on 
SDS-PAGE. I read somewhere that sometimes mutations can alter the interactions between 
the protein and SDS, resulting in altered apparent mobilities on SDS-PAGE. If anyone 
could confirm this or provide me with a reference I would be very grateful.


Thanks 

Dave			      E-mail: CLARKE at IGMORS.U-PSUD.FR