alan friedman wrote:
>> Does anyone know whether Factor Xa from non-humans can
> be used for cleavage of Factor Xa linked fusion proteins?
> (In the middle of corn country cow blood is easy to come by.)
> Are there protocols for making non-human Factor Xa?
> Thanks.
>> alan friedman
Alan,
As a graduate student, I purified about 50 mg Factor X from 6 liters of
bovine blood by following (exactly) the protocol given by Nagai and
Thorgersen [Meth. Enzymol. 153, 461-481 (1987)]. The procedure is
straightforward and extremely cost-effective, and you end up with
high-quality enzyme.
Fred Hughson
