Dear Netter's
Does anybody know the best methods to isoelectrofocusing proteins with or
without urea?
And what are the best ways to detect them by immunoblotting?
I have many problems to transfer proteins very well. It seemed that I need
5 or 10 more proteins to see something on the transfer membrane.
What membrane do I use?
What are the conditions for semy-dry electrotransfer?
Has the gel to be washed before transfer ...
Even my proteins are radiolabelled (14C) I don't see a very good signal as
I have with SDS-PAGE. I think the proteins pass through the membrane?
Than you
Joel
