>>>>>>>POLYMERSE CHAIN REACTION (PCR) APPLICATIONS/
CYCLE DNA SEQUENCING<<<<<<<
American Type Culture Collection (ATCC)
April 21-24, 1997, Rockville, MD
A four-day, laboratory-intensive, course covering a broad range of
applications of the polymerase chain reaction (PCR) for addressing
biological problems. Included will be a DNA sequencing experiment using a
thermostable DNA polymerase and a temperature
cycling protocol (cycle sequencing). The workshop will provide
introductory and intermediate level instruction in the application,
procedures and trouble-shooting for this extremely useful and versatile
technology.
A basic knowledge of nucleic acid laboratory procedures is helpful, but
not necessary. Approximately 75% of the course is devoted to laboratory
instruction while 25% is lecture-oriented. An extensive manual with
protocols and procedures is provided for
each participant.
Lecture topics will include PCR Reaction Basics, Optimizing and
Troubleshooting the PCR Reaction, DNA Sequencing, PCR Analysis of
Ribosomal DNA to Determine Taxonomic Relatedness, PCR Fingerprinting, PCR
in the Diagnosis of Infectious and Genetic Disease, and PCR and
Recombinant DNA Methodology. Time will be
structured for presentation and discussion of workshop participants' PCR
applications/problems.
The workshop is intended to be a laboratory-intensive course. Laboratory
exercises will include: 1) PCR Diagnosis of Sickle Cell Disease; 2)
Fingerprint Analysis of the DNA of Human Individuals; 3) Relatedness of
Organisms by Analysis of Amplified Ribosomal DNA; 4) Amplification from
mRNA; 5) Optimization of PCR Reactions; 6) Labeling of Hybridization
Probes by PCR; 7) Cycle DNA Sequencing; and 8) Fingerprinting of
Microorganisms by RAPD and Inter-repeat PCR. Results will be analyzed and
discussed.
Faculty: William Nierman, Ph.D. (Workshop Director), Director, ATCC
Program in Molecular Biology and Virology; Donna Maglott, Ph.D., Research
Scientist; Yvonne Reid, Ph.D., Collection Scientist; Francis Molina,
Ph.D., Research Scientist; Tamara
Feldblyum, M.S., Collection Scientist.
* The polymerase chain reaction (PCR) process is covered by patents owned
by Hoffmann-La Roche. Use of the PCR process requires a license.
Limited to 25 participants
FEE: $1,195.00
2.8 CEUs
**For a full schedule and on-line registration, please visit our Web site:
http://www.atcc.org/workshops/workshop.html
**Or request a brochure, which includes a full schedule and registration
form from: kcarter at atcc.org
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Ken Carter ^
American Type Culture Collection / l \ (301) 231-5525
12301 Parklawn Drive /___l___\__ fax:(301) 816-4364
Rockville, Maryland 20852 \______/ kcarter at atcc.org
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