In article <19970115221601.RAA20724 at ladder01.news.aol.com>,
jlk1117440 at aol.com (Jlk1117440) wrote:
> I would like to extract a protein (~26 kd) from an SDS-PAGE gel. What is
> the best way to extract the protein, and what yield can I expect? Jim
> Knoetgen,MD
I think electroelution is the best alternative. You might get even up to
100 %. Passive Elution has a yield of 50-80% in my opinion.
The best way to electroelute is to buy a commercially availabla
electroeluter, for example from Biorad.
In case your choice would be the passive elution (very cheap), mail me and
i'll supply you with some references.
Ralf
--
Ralf Spenneberg
Institute for Medical Biochemistry
Universitaet Muenster 0251 83 6722
Von Esmarchstr 56 48149 Muenster Germany
