derek12 at ix.netcom.com(Russell Lamar Howard) wrote:
>I need some info on TCA precip. I'm fairly new at it and would like a
>"generic" method to using it. Also, what is a SDS_page
SDS-PAGE stands for Sodium Dodecyl Sulfate PolyAcrylamide Gel
Electrophoresis, an analytical method introduced by Laemli some 20 years
ago to separate proteins based on differences in molecular weight. Get a
book on protein chemistry or practical biochemistry to learn all the
details.
If you are developing a new method, I would advice against TCA
precipitation and recommend chloroform/methanol precipitation instead.
The method was published by Wessel & Fluegge in Anal. Biochem., somewhere
in the mid 80s. Protein recovery is much better.
