In article <32D42CE3.3958 at amgen.com>, jphilo at amgen.com wrote:
> Ronald Smulders wrote:
I missed the original post, but have just been discussing this kind of
situation with my student. Is the absolute value of the ellipticity
increasing or decreasing? If it is decreasing, I would suggest aggregation
leading to light scattering. If the absolute value is increasing, perhaps
more beta structure is being formed. Have you calculated the % beta etc.
under the two circumstances? There was a paper by Greenfield within the
last couple of years in Anal. Biochem. that compares several algorithms,
some work better than others with predominately beta proteins.
Ellie Brown
ERRC-USDA
Wyndmoor, PA 19038
