Dear colleagues,
We've isolated and cDNA encoding a protein which should have a
weight of 25 kD dased on computer program calculation. However, after we
did Western blot using antibodies specific to this protein we got a 33kD
band on SDS-PAGE (under reduced conditions). Although this protein is a
glycoprotein, it shouldn't change so much since only one glyco-site was
found. Does any one can explain this for me? Any suggestions are highly
appreciated.
Please respond by e-mail.
Wenjin Yu
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|| Wen-Jin Yu * E-Mail: wyu at uoguelph.ca ||
|| Dept. of Botany * Tel.(home): (519)836-2868 ||
|| University of Guelph * Tel.(work): (519)824-4120, x4779 ||
|| Guelph, Ont. Canada * Fax: (519)767-1991 ||
|| N1G 2W1 * URL: http://www.uoguelph.ca/~wyu ||
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