Lee, Ji Hyun wrote:
>> When much proteins of large molecular weight co-exist, do proteins of
> small molecular weights tend to be underestimated on SDS-PAGE? In other
> words, compared to their absolute contents their bands look light in
> mixture of very much of other proteins but when only they are SDS-PAGEd
> their bands look dark despite of the same contents.
> Is it possible? What reference is helpful to me?
> Any comments will be appreciated.
>> Thank you.
Though I cannot give you a literature reference, from my own experience
I think this to be true.
Achim
