In article <32CED947.24E1 at jcu.edu.au>,
lachlan harris <lachlan.harris at jcu.edu.au> wrote:
>Can anyone help me with this problem? I have a pure microbial protein
>which when run on an SDS non-reduced (ie no mercaptoethanol) PAGE gel
>runs at approximately 50 KDa. When the same protein is run under
>SDS-reduced conditions two subunits appear- one running at approx 40 KDa
>and the other at approx 60 KDa.
>>It was my understanding that the two subunits should add up to be the
>same size as the protein run under SDS-non reduced conditons. But this
>isn't happening. Does anyone know why?
I don't believe this is a subunit problem. The subunit can't be larger than the
original protein. I believe you are not geting a complete reduction but not
knowing the conditons you are using I can't say for sure. Try more sever
reduction conditions.
