Rainer Hofmann wrote:
newera at plaza.snu.ac.kr () wrote:
>Some or probably quite many air bubbles seem to be trapped in my
HPLC column: 18C reverse phase column, pore size 7um.
>Could you give me any suggestions to remedy this problem as quickly
as possible?
>I am much disappointed not to be able to proceed my experiments any
more.
I guess the problem is poor degassing of solvents. At pressure
levels
in a hplc column there aren't any free gas bubbles. But when
pressure
drops at the column's outlet gas is set free again and bubbles
disturb
detection.
You should use Helium to purge solvents and to keep them under a
Helium atmosphere. Online degassing systems are also available.
--
Rainer Hofmann
rhofmann at freiburg.netsurf.de
Kalkackerweg 20 fax: +49 761
4764136
79111 Freiburg phone: +49 761
4764135
Germany
Hi:
You can degas solvents under sonicator and use air pump to remove air
bubles in solvent.
Try it, it works to me many years!!
Lin Chung-Yen
Institute of Zoology,
National Taiwan Univ.
Taiwan
