staining of proteins with EOSIN Y

Dr E. Buxbaum EB15 at le.ac.uk
Tue Jul 30 05:54:24 EST 1996

I have done it a couple of times. Instead of 1% eosin I used 0.1%, for 
destaining I used the same protokoll as for coomassie, i. e. several 
changes of 40% MeOH, 5% HAc, rest water. A "dirty trick" is to destain 
with a dilute solution of Tris (eosin is an acid, the Tris-salt is much 
more soluble), but you have to be carefull to stop the reaction by 
immersion in 5% HAc before the gel is destained completely. 

In general, however, I prefer Calcon carboxylic acid over eosin and 
coomassie. The stuff is used as a direct replacement of CBB, giving 
improved sensitivity. 

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