I have done it a couple of times. Instead of 1% eosin I used 0.1%, for
destaining I used the same protokoll as for coomassie, i. e. several
changes of 40% MeOH, 5% HAc, rest water. A "dirty trick" is to destain
with a dilute solution of Tris (eosin is an acid, the Tris-salt is much
more soluble), but you have to be carefull to stop the reaction by
immersion in 5% HAc before the gel is destained completely.
In general, however, I prefer Calcon carboxylic acid over eosin and
coomassie. The stuff is used as a direct replacement of CBB, giving
improved sensitivity.
