help HIS Fab purification!

jose luis toran jltoran at SAMBA.CNB.UAM.ES
Wed Jul 24 13:14:29 EST 1996

dear netters,
I try to purify a  his-tagged fab  cloned in pComb3 h from a 
periplasmic extract, any had a satifactorial protocol to 
extract and eluted on Ni-NTA resin 

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