elution of an his-tag protein

Falson falson at verlaine.saclay.cea.fr
Mon Jul 22 10:37:08 EST 1996

Keld Sorensen wrote:
> Try elution with 100 mM EDTA
> Keld.

thank you keld, but I forgot to say that our protein is denaturated 
by EDTA or EGTA once solubilized...hum

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