protein electrophoresis

Achim Recktenwald achim at ibex.ca
Mon Jul 22 15:16:57 EST 1996

Ayari Sahbi wrote:
> Hi
> I know that a dimeric protein will separate during SDS-PAGE, but is this
> dimer will separate in his two subunits when it is in his native form,
> during non-dissociating PAGE?
>  Kind regards,
>  Sahbi Ayari.

Not necessarily. If the subunits are crosslinked by SS-bridges, they will definitely 
stay together under non-reducing conditions. But I know of at least one example, 
L-leucin dehydrogenase from Bacillus subtilis, where the electric field during native 
PAGE is not strong enough to disrupt the bond between the subunits caused by hydrophobic 
or salt-bridges.


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