Ayari Sahbi wrote:
>> Hi
>> I know that a dimeric protein will separate during SDS-PAGE, but is this
> dimer will separate in his two subunits when it is in his native form,
> during non-dissociating PAGE?
>> Kind regards,
> Sahbi Ayari.
Not necessarily. If the subunits are crosslinked by SS-bridges, they will definitely
stay together under non-reducing conditions. But I know of at least one example,
L-leucin dehydrogenase from Bacillus subtilis, where the electric field during native
PAGE is not strong enough to disrupt the bond between the subunits caused by hydrophobic
or salt-bridges.
Achim
