Hello,
I am attempting to run membrane proteins on a 2-D gel, but am
experiencing no separation in the IEF first dimension. I have tried using
0.5% Triton X-100 in the solubilisation buffer, and have now been
informed that minimal SDS/DTT might help also. Does anyone know about the
use of chloryl hydrate to help in solubilisation/inhibition of
aggregation, if that is what is happening ?
Any help glady accepted,
Laurence Barker.
The Horticultural and Food Research Institute of New Zealand,
Mt Albert Research Centre,
Auckland,
New Zealand
l.barker at auckland.ac.nz
