IUBio

gel staining

Dima Klenchin klenchin at macc.wisc.edu
Fri Jul 19 11:50:59 EST 1996


In article <31F02CB0.7E58 at smail.rrz.uni-koeln.de>,
   a2279762 at SMAIL1.RRZ.UNI-KOELN.DE (Norbert Tennagels) wrote:
->hello folks,
->
->I would like to stain a SDS gel under alkaline or acidic conditions. (pH
->14 or pH 1).
->Does anybody know a good method which does not destroy the gel and
->remove the alkaline or acidic reagents after staining?

Won't standard coomasie (10% Acetate, 40% MeOH) give you pH ~ 1.0???
After that - water ot anything else. 

- Dima





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