how to cleave His-tag

Jeff Seale seale at bioc02.uthscsa.edu
Wed Jul 17 10:03:31 EST 1996

TEV protease from GIBCO/BRL (Life Technologies) works very well for
us. In addition, the protease is engineered to contain a His-tag
as well, so it is easily removed when the cleaved protein is re-purified.


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