hello,
I am looking for the proteins containing in saliva of aphids. I try to
identify these proteins in gel electrophoresis, but untill now I always have a
strong smear during the migration, I have used several reductors (urea,
mercapto, DTT, iodoacetamide) without any results, the smear is still
appearing.
Any informations about a procedure to remove this smear would be greatly
apreciated,
Responses can be emailed to the below address.
Thanks,
Dr. A. Cherqui
Dept. of Entomology
Wageningen Agricultural University
Binnenhaven 7, 6709 PD
Wageningen, the Netherlands
Tel +31-317-485118 Fax +31-317-484821
Email: <anas cherqui at medew.ento.wau.nl>