Thomas Wilkinson (wilkinst at deft.cc.purdue.edu) wrote:
: Newsgroups: bionet.molecules.peptides
: Subject: gel electrophoresis of peptides...
: Summary:
: Followup-To:
: Distribution: world
: Organization: Purdue University Computing Center
: Keywords:
: Hello,
: I was wondering if somebody had some experience with SDS gel electrophoresis of
: peptides. I have a sample that I would like to analyze to see if it contains
: a short (12-residue) peptide, molecular weight = 1462.
: The sample is not some mixture of many species...the sample to be analyzed
: either has the 12-mer, or else the sample contains no peptide whatsoever. Thus,
: this gel should be really easy to interpret. I also have a similar sample that
: I would like to analyze for peptide content, except in this case I am looking
: for the presence or absence of a 33-residue peptide (MW = 3487).
: I would like to run these samples out on an SDS gel, and then coomassie-stain.
: My question is that I don't know what the optimal percentage of polyacrylamide
: would be for this task. What percent gel is best? Is gel electrophoresis of
: peptides commonly done? Your suggestions are much appreciated.
: thanks,
: tom
No experience with anything like that, but I would recommend Novex
(800)456-6839, a company that sells equipment and supplies including
precast gels
using the tris-tricine gel system that claims to be able to resolve
peptides about as small as those you mention. Good luck
--
Steve Driska, Physiology Department, Temple University Medical School
Philadelphia, PA 19140 USA (215) 707-3283
driska at astro.ocis.temple.edu
If I could think of something witty and clever to say, it would go right
here ----->> .........................
