gel electrophoresis of peptides...

Thomas Wilkinson wilkinst at deft.cc.purdue.edu
Wed Jul 10 17:11:23 EST 1996

Newsgroups: bionet.molecules.peptides
Subject: gel electrophoresis of peptides...
Distribution: world
Organization: Purdue University Computing Center


I was wondering if somebody had some experience with SDS gel electrophoresis of
peptides.  I have a sample that I would like to analyze to see if it contains
a short (12-residue) peptide, molecular weight = 1462.

The sample is not some mixture of many species...the sample to be analyzed
either has the 12-mer, or else the sample contains no peptide whatsoever.  Thus,
this gel should be really easy to interpret.  I also have a similar sample that
I would like to analyze for peptide content, except in this case I am looking
for the presence or absence of a 33-residue peptide (MW = 3487).

I would like to run these samples out on an SDS gel, and then coomassie-stain.  
My question is that I don't know what the optimal percentage of polyacrylamide 
would be for this task.  What percent gel is best?  Is gel electrophoresis of
peptides commonly done?  Your suggestions are much appreciated.



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