Can someone suggest a reference for affinity chromatography of whole antiserum
to adsorb unwanted specificities? For example, I have rabbit antiserum
against whole deer serum. I want to adsorb out the components which cross
react with bovine proteins. I was thinking of using epoxy-activated Sepharose
to bind with whole bovine serum, reacting with the anti-deer on a column,
washing with PBS and eluting with high pH buffer.
Any references or comments would be very welcome.
Thanks.
Bob Hoesch
U.S. Fish & Wildlife Forensic Laboratory
Ashland, OR
