Alkaline Phosphatase Assay

Becky Norum bnorum at lynx.dac.neu.edu
Mon Jul 8 21:25:08 EST 1996

I am working on Alkaline Phosphatase and am searching for the kinetic
assay that will give the highest specific activity.  We generally use
the Sigma Alkaline Phosphatase Assay but are trying to get a kinetic
assay using DEA as the buffer and PNPP as the substrate to work. 
Unfortunately, everytime I perform this assay the specific activities
obtained are about three-quarters of what we obtain with the Sigma assay
when theoretically the DEA assay should yield higher results.  If anyone
has performed the DEA assay and is willing to talk about their procedure
with ,e I would appreciate it.  Responses can be emailed to the below
address.  Thanks,

Becky Lynn			
bnorum at lynx.dac.neu.edu  'essie'
All the darkness of the world cannot suppress the light from one lit

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