In article <federici-0407961606580001 at federici.cochin.inserm.fr>,
federici at icgm.cochin.inserm.fr (Federici) wrote:
> But, due to their amino acid content, some E.coli proteins are also bound
> to the Ni-column, and disturb the purification of the recombinant protein.
> Has anybody identified E.coli proteins which are retained during
> purification using a Ni-column?
> I would also be very interested in references describing this subset of
> E.coli proteins.
Check out Wulfing et al Journal Biol Chem 269(4) 2895-2901. They discuss
a protein (WHP) which does bind to a Ni-column.
--
Scott McMahan
mcmahan at oncology.wisc.edu
