Hello!
Im working on the major yolk proteins of two european freshwater teleosts.
One method is SDS-gelelektrophoresis. So I now that one of the major yolk
proteins is a highly phosphorylated protein named phosvitin. To stain it
on the acrylamidgels, I cnat use coomassie, because it wont stain
phosphoproteins. So I use the method of Cutting and Roth (1973), staining
them with methylgreen. But....I doesnt work anyway.
Is there anyone "outside" who will know a recipe for staining
phosphoproteins on acrylamidgels, or could give me any hint?
Thanks in advance,
Jens Krieger.
--
/~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\
| Jens Krieger "Solange die Alpen noch stehen, |
| TU Dresden, Zoology wird es keine |
| Tel.:+49.351.463.5465 Gerechtigkeit geben."|
| |
|krieger at rcs.urz.tu-dresden.de H.Achternbusch |
\ krieger at mibm.ruf.uni-freiburg.de /
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